Director:
Dr. Christine Karim,
Research Assistant Professor
Technical
support:
Zhiwen Zhang,
Assistant Scientist
This facility provides custom synthesis, purification, and characterization of peptides. We specialize in long peptides (> 30 amino acids) that offer special challenges, including highly hydrophobic or amphiphilic membrane proteins. We also have special expertise in synthesis of peptides labeled with fluorescent or paramagnetic (spin label) probes. Solid-phase peptide synthesis is carried out using a PE Biosystem Pioneer Peptide Synthesis System [photo]. Purification is carried out using Beckman System Gold HPLC [photo]. Samples are fully characterized by mass spectrometry and amino acid analysis, sometimes supplemented by partial amino acid sequencing.