Former Lab Member

Ingrid Brust-Mascher

Office:     BSBE 1-138
Phone: (612) 625-6480
Fax: (612) 624-0632
Mail:




Ingrid Brust-Mascher 
Department of Biochemistry 
University of Minnesota Medical School 
4-225 Millard Hall 
435 Delaware St SE 
Minneapolis, MN 55455
Email: ibm@ddt.biochem.umn.edu



Research Interests:

The orientation and mobility of the myosin head during muscle contraction.

I am using spectroscopic techniques to study the orientation and mobility of the myosin head during muscle contraction. A phosphorescent probe or a spin label is attached to the myosin regulatory light chain and the native light chain is replaced by the labeled light chain. Phosphorescence anisotropy in rabbit skeletal myofibrils probes myosin mobility and electron paramagnetic resonance in scallop fiber bundles (done in collaboration with Sampath Ramachandran and Josh Baker) gives information about both orientation and mobility. 

 

Publications:

1. Feder T.J., I. Brust-Mascher, J.P. Slattery, B. Baird and W.W. Webb. 1996. Constrained diffusion or immobile fraction on the cell surface: a new interpretation. Biophys. J. 70:2767-2773. 

Abstracts 

1. Brust-Mascher, I., T.J. Feder, J.P. Slattery, B. Baird and W.W. Webb. 1992. Constrained Diffusion or Immobile Fraction on the Cell Surface: a new Interpretation. Mol. Biol. Cell 3:306a. 

2. Brust-Mascher, I., T.J. Feder, J.P. Slattery, B. Baird and W.W. Webb. 1993. FPR Data on Mobility of Cell Surface Proteins Reevaluated in terms of Temporally Constrained Molecular Motions. Biophys. J. 64:A354. 

3. Williams, R.M., I. Brust-Mascher, D.W. Piston and W.W. Webb. 1993. Calcium Activity Gradients in Motile Fish Keratocytes measured by Two-Photon Excitation Fluorescence Microscopy. Biophys. J. 64:A367. 

4. Ghislain, L.P., I. Brust-Mascher and W.W. Webb. 1993. Force and Membrane Compliance Measurements using an Optical Trap and Laser Interferometry. Biophys. J. 64:A109. 

5. Brust-Mascher, I., R.M. Williams and W.W. Webb. 1993. Three-dimensional Measurements of Calcium Distributions in Motile Fish Epidermal Cells using Two-Photon Excitation Fluorescence Microscopy. Mol. Biol. Cell 4:166a. 

6. Brust-Mascher, I., R.M. Williams and W.W. Webb. 1994. Calcium Distributions in Motile Electrotactic Fish Keratocytes Measured by Two-Photon Excited Fluorescence Microscopy. Biophys. J. 66:A411. 

7. Brust-Mascher, I., R.M. Williams and W.W. Webb. 1994. Fast Calcium Waves Induced in Electrotactic Fish Keratocytes by a Pulsed Electric Field. Mol. Biol. Cell 5:293a. 

8. Brust-Mascher, I., R.M. Williams and W.W. Webb. 1995. Calcium Heterogeneities in Fish Keratocytes Turning in Electric Fields. Biophys. J. 68:A282.

 

Techniques:

Phosphorescence Anisotropy

Electron Paramagnetic Resonance

 


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