Microland – time-resolved phosphorimeter designed in DDT lab. It is mostly
focused on observing the motions in large protein assemblies, such as membranes
and muscle filaments measuring transient phosphorescence anisotropy in range 0.1-10 ms.
The phosphorescence is excited by second harmonic of diode pulped passively Q-switched
YAG laser FDSS532-150 (
CryLas Laser Systems, Berlin
Germany). Phosphorescence signal is measured by 14 bits Compuscope 14100 digitizer board
(
Gage Applied Technologies,
Lockport IL). Transient phosphorescence anisotropy is calculated using data measured
for vertical and horizontal position of emission polarizer. Temperature of sample can be
controlled by Peltier temperature controller. Our software make all process
of data acquisition and analysis fully automated.
Recently nitrogen laser NL100
(
Stanford Research Systems, Sunnyvale, Canada) was
integrated in instrument. The laser is used as excitation source for experiments
with terbium chelate.