Fargoland
This is time-resolved fluorimeter based on direct digitizing of luminescence
signal excited by short light pulse. We use second or third harmonic of passive
Q-switched YAG microchip laser as excitation source and 8 Gs/sec digitizer DS252
( Acqiris, Geneva, Switzerland). This instrument
requires less than second for data acquisition and lets recover information about
luminescence with lifetime as short as 0.1 ns. Basically we have two instruments what
share digitizer and controlled computer:
Stand-alone instrument based on optical table and uses optical filter for
wavelengths separation. A flow-through temperature bath is coupled to the instrument,
allowing control of the temperature during the measurements
Accessory for Eclipse fluorimeter what fits in Eclipse
sample compartment and uses its emission monochromator. The accessory is equipped with Peltier
temperature-controlled cuvette holder TLC 50 (Quantum Northwest,
Spokane WA) with magnetic stirring and four open windows for fluorescence.
Instrument controlling software can carry out simple single waveform experiments or
automatically create 3-D experiments with many waveforms as function of emission wavelength,
emission polarizer orientation, and delay time. For delay time based experiments stand-alone
instrument has stopped-flow accessory what is customized SFA-20 mixing accessory
( Hi-Tech Scientific, Bradford on Avon, United Kingdom).
Moreover user can manually perform series of experiments based on titration, temperature
or another conditions.
 Recently stopped-flow module SFM-20
( Bio-Logic, Claix, France) was integrated in instrument.
It consists of two step motor driven syringes and optical observation head. The original light collection
and detection systems were designed for this accessory. This accessory can be used for double-kinetic
experiment that is a simultaneous detection of luminescence changes in nanosecond and millisecond
time scales. Because of efficient design it has stopped-flow dead time only about 2 ms.
Our state-of-art data analysis software is designed to globally analyze virtually infinite
number of waveforms using extremely sophisticated fitting models. Model is designed by user
as a combination of processes such as light emission, quenching, resonance energy transfer,
rotation molecule diffusion, chemical reactions, etc.
 Fargoland schedule
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